||Human Hemopexin produced in Human plasma having a molecular mass of 70 kDa.
||HPX (or haemopexin) is a plasma protein that binds heme with the highest affinity of any known protein. HPX is generally expressed in liver, and belongs to acute phase reactants, the synthesis of which is induced after inflammation. Heme is potentially very toxic because of its ability to intercalate into lipid membrane and to generate hydroxyl radicals. HPXμs function of scavenging the heme released or lost by the turnover of heme proteins such as hemoglobin defends the body from the oxidative damage that free heme can cause. Additionally, HPX discharges its bound ligand for internalisation upon interacting with a specific receptor located on the surface of liver cells. This HPX function is in order to preserve the body"s iron. HPXμs levels in the serum are an indication of how much heme is present in the blood. Low HPX levels show that there is a lot of it in the serum. For that reason, low HPX levels indicate that there has been considerable degradation of heme containing compounds - mainly hemoglobin, it indicates hemolysis and low HPX levels are therefore one of the diagnostic features of a hemolytic anemia. Itμs a Haem binding protein used in the assessment of intravascular haemolysis in conjunction with haptoglobin.
||Lyophilised from 11.9mM phosphate buffer pH- 7.4, 137mM NaI and 2.7mM KCl.
||Greater than 95.0%.
||Sterile Filtered White lyophilized (freeze-dried) powder.
||It is recommended to reconstitute the lyophilized HPX in phosphate buffer, pH >7.0 containing 0.15M NaCl.
||Human HPX although stable at room temperature for 2 weeks, should be stored at -20°C.