Recombinant Human IGHG1 protein

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Recombinant Human IGHG1 protein

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Cat.No. : IGHG1-4763H
Product Overview : Recombinant Human IGHG1 protein (Glu99-Lys330) carries no tag and was expressed in human 293 cells (HEK293).
Description : Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
Source : HEK293
Species : Human
Form : Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 26 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : 232
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of IGHG1-4763H :

Human IgG Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of IGHG1-4763H :

Immobilized Human CD64, His Tag at 10μg/mL (100 μL/well) can bind Human IgG Fc, Tag Free with a linear range of 7-41 ng/mL (QC tested).

Gene Name : IGHG1
Official Symbol : IGHG1
Synonyms : IGHG1; Immunoglobulin heavy constant gamma 1(G1m marker)
Gene ID : 3500
MIM : 147100
UniProt ID : P01857
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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