Active Recombinant Human Insulin

• Cat.No.: INS-256H
• Product Overview: INS Human Recombinant produced in Yeast is a two chain, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. Zinc content was found to be 0.4%. INS is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: INS-256H
• Description: INS decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
• Source: Human
• Host: Saccharomyces cerevisiae.
• Form: The protein was lyophilized from 50mM Sodium Chloride solution.
• Purity: Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Bio-activity: 27 units/mg. INS has been evaluated in cell culture (Human Foreskin Fibroblasts). The effective concentration range for use in defined media is 1-5ug/ml. However, the optimum concentration range for a particular application must be determined by the investigator.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized INS in PBS pH-7.5.
• Storage: Lyophilized INS should be stored at 4°C. Upon reconstitution INS should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: INS insulin [ Homo sapiens ]
• Official Symbol: INS
• Synonyms: INS; insulin; LPR; IRDN; IDDM2; MODY10; proinsulin; OTTHUMP00000011161; OTTHUMP00000011162; OTTHUMP00000196036; OTTHUMP00000196038; OTTHUMP00000217519
• Gene ID: 3630
• mRNA Refseq: NM_000207
• Protein Refseq: NP_000198
• MIM: 176730
• UniProt ID: P01308
• Chromosome Location: 11p15.5
• Pathway: ATF-2 transcription factor network; ATF-2 transcription factor network; ATF-2 transcription factor network; Amyloids; Arf6 trafficking events; Diabetes pathways; Downstream signaling of activated FGFR; Folate Metabolism; IRS activation; IRS-mediated signaling; IRS-related events; Insulin Pathway; Insulin Synthesis and Processing; Insulin signaling pathway; PI-3K cascade
• Function: hormone activity; insulin receptor binding; insulin-like growth factor receptor binding; protein binding