Recombinant Human Insulin


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Recombinant Human Insulin

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Cat. No. : INS-256H
Product Overview : INS Human Recombinant produced in Yeast is a two chain, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. Zinc content was found to be 0.4%. INS is purified by proprietary chromatographic techniques.
Description : INS decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Source : Human
Host : Saccharomyces cerevisiae.
Form : The protein was lyophilized from 50mM Sodium Chloride solution.
Purity : Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Bio-activity : 27 units/mg. INS has been evaluated in cell culture (Human Foreskin Fibroblasts). The effective concentration range for use in defined media is 1-5ug/ml. However, the optimum concentration range for a particular application must be determined by the investigator.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility : It is recommended to reconstitute the lyophilized INS in PBS pH-7.5.
Storage : Lyophilized INS should be stored at 4°C. Upon reconstitution INS should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Computer-generatedimage of six insulin molecules assembled in a hexamer, highlighting thethreefold symmetry, the zinc ionsholding it together, and the histidine residuesinvolved in zinc binding. Insulin is stored in the body as a hexamer, whilethe active form is the monomer. :

Gene Name : INS insulin [ Homo sapiens ]
Official Symbol : INS
Synonyms : INS; insulin; LPR; IRDN; IDDM2; MODY10; proinsulin; OTTHUMP00000011161; OTTHUMP00000011162; OTTHUMP00000196036; OTTHUMP00000196038; OTTHUMP00000217519
Gene ID : 3630
mRNA Refseq : NM_000207
Protein Refseq : NP_000198
MIM : 176730
UniProt ID : P01308
Chromosome Location : 11p15.5
Pathway : ATF-2 transcription factor network; ATF-2 transcription factor network; ATF-2 transcription factor network; Amyloids; Arf6 trafficking events; Diabetes pathways; Downstream signaling of activated FGFR; Folate Metabolism; IRS activation; IRS-mediated signaling; IRS-related events; Insulin Pathway; Insulin Synthesis and Processing; Insulin signaling pathway; PI-3K cascade
Function : hormone activity; insulin receptor binding; insulin-like growth factor receptor binding; protein binding

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