||Recombinant human NACA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||NACA is member of the nascent polypeptide associated complex (NAC) alpha subunit family that participate in preventing inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Most NACA proteins localize to both nucleus as well as cytoplasm, and contain NAC-A/B (NAC-alpha/beta) and UBA (ubiquitin-associated) domains. The UBA domain is associated with proteins involved in the ubiquitin-proteasome pathway for protein degradation.
||Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.
||25.5 kDa (235aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
||> 95 % by SDS - PAGE
||1 mg/ml (determined by BCA)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQATTQQ AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVTG VTRVTIRKSK NILFVITKPD VYKSPASDTY IVFGEAKIED LSQQAQLAAA EKFKVQGEAV SNIQENTQTP TVQEESEEEE VDETGVEVKD IELVMSQANV SRAKAVRALK NNSNDIVNAI MELTM
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.