Recombinant Human Phosphoglycerate Kinase 1, His-tagged

• Cat.No.: PGK1-915H
• Product Overview: PGK1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 437 amino acids ( 1-417 a.a.) and having a molecular mass of 46.8 kDa. PGK1 is fused to 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Specification

• Cat. No.: PGK1-915H
• Description: PGK1 is an X-linked enzyme that has a major role in the glycolytic pathway. PGK1 is a glycolytic enzyme which catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating an ATP molecule.
• Source: Human
• Host: Escherichia Coli.
• Form: The PGK1 solution containing 20 mM Tris ( pH 8.0 ), 10 % Glycerol and 1mM DTT.
• Purity: Greater than 95.0 % as determined by SDS-PAGE.
• Physical Appearance: Sterile filtered colorless solution.
• Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI
• Storage: PGK1 although stable at 4°C for 1 week, should be stored below -18°C. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA). Please prevent freeze thaw cycles.

Gene Information

• Gene Name: PGK1 phosphoglycerate kinase 1 [ Homo sapiens ]
• Official Symbol: PGK1
• Synonyms: PGK1; phosphoglycerate kinase 1; PGKA; MIG10; MGC8947; MGC117307; MGC142128; phosphoglycerate kinase 1; PRP 2; OTTHUMP00000023595; primer recognition protein 2; cell migration-inducing gene 10 protein
• Gene ID: 5230
• mRNA Refseq: NM_000291
• Protein Refseq: NP_000282
• MIM: 311800
• UniProt ID: P00558
• Chromosome Location: Xq13.3
• Pathway: Gluconeogenesis; Glucose metabolism; Glycolysis; Glycolysis / Gluconeogenesis; Glycolysis and Gluconeogenesis; HIF-1-alpha transcription factor network; Metabolic pathways; Metabolism of carbohydrates
• Function: ATP binding; nucleotide binding; phosphoglycerate kinase activity; transferase activity