Recombinant Mouse Pla2g1b protein(Met1-Cys146), His-tagged

• Cat.No.: Pla2g1b-1185M
• Product Overview: Recombinant Mouse PLA2G1B (NP_035237.1) extracellular domain (Met 1-Cys 146) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Cys146
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant mouse PLA2G1B consists of 142 amino acids and has a predicted molecular mass of 16.3 kDa. rmPLA2G1B migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Epithelial-Cell-Derived Phospholipase A 2 Group 1B Is an Endogenous Anthelmintic. (2017)

Gene Information

• Gene Name: Pla2g1b phospholipase A2, group IB, pancreas [ Mus musculus ]
• Official Symbol: Pla2g1b
• Synonyms: PLA2G1B; phospholipase A2, group IB, pancreas; phospholipase A2; PLA2-Ib; group IB phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B; Pla2a; sPLA2IB; MGC6679
• Gene ID: 18778
• mRNA Refseq: NM_011107
• Protein Refseq: NP_035237