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Recombinant Human Proteasome (Prosome, Macropain) Subunit, Beta Type, 4, His-tagged

Cat. No.: PSMB4-1541H
Product Overview: Recombinant human PSMB4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description: Proteasome subunit beta type-4, also known as PSMB4, belonged to the peptidase T1B family. The 20S Proteasome chamber contains alpha subunits (which are structural) and beta subunits (which are predominantly catalytic). The outer two rings in the proteasome consist of seven alpha subunits each and the inner two rings each consist of seven beta subunits. PSMB4 is a beta subunit of the 20S Proteasome. It is distributed throughout eukaryotic cells at a high concentration and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
Molecular Weight: 26.6 kDa (240aa), confirmed by MALDI-TOF
Purity: > 90% by SDS - PAGE
Concentration: 0.5 mg/ml (determined by Bradford assay)
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MTQNPMVTGT SVLGVKFEGG VVIAADMLGS YGSLARFRNI SRIMRVNNST MLGASGDYAD FQYLKQVLGQ MVIDEELLGD GHSYSPRAIH SWLTRAMYSR RSKMNPLWNT MVIGGYADGE SFLGYVDMLG VAYEAPSLAT GYGAYLAQPL LREVLEKQPV LSQTEARDLV ERCMRVLYYR DARSYNRFQI ATVTEKGVEI EGPLSTETNW DIAHMISGFE
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways: Proteasome
Gene Name: PSMB4 proteasome (prosome, macropain) subunit, beta type, 4 [ Homo sapiens ]
Official Symbol: PSMB4
Synonyms: PSMB4; proteasome (prosome, macropain) subunit, beta type, 4; HN3; HsN3; PROS26; PROS-26; proteasome subunit beta type-4; hsBPROS26; proteasome chain 3; macropain beta chain; proteasome beta chain; 26 kDa prosomal protein; proteasome subunit HsN3; proteasome subunit, beta type, 4; multicatalytic endopeptidase complex beta chain; EC 3.4.25.1
Gene ID: 5692
mRNA Refseq: NM_002796
Protein Refseq: NP_002787
MIM: 602177
UniProt ID: P28070
Chromosome Location: 1q21
Function: lipopolysaccharide binding; peptidase activity; threonine-type endopeptidase activity

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