||Recombinant human Rab18 protein, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||RAB18 is a member of a family of Ras-related small GTPase that is lipid-anchored to the cytoplasmic side of the cell membrane. It plays a role in apical recycling and endocytosis and is also thought to be involved in protein transport between early endosomes and the plasma membrane.
||Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT.
||24.5 kDa (221aa)
||> 90 % by SDS - PAGE
||1 mg/ml (determined by Bradford)
|Sequences of amino acids:
||MASMTGGQQM GRGSHMDEDV LTTLKILIIG ESGVGKSSLL LRFTDDTFDP ELAATIGVDF KVKTISVDGN KAKLAIWDTA GQERFRTLTP SYYRGAQGVI LVYDVTRRDT FVKLDNWLNE LETYCTRNDI VNMLVGNKID KENREVDRNE GLKFARKHSM LFIEASAKTC DGVQCAFEEL VEKIIQTPGL WESENQNKGV KLSHREEGQG GGACGGYCSV L
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.