||Recombinant human RAB34 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||RAB34, also known as ras-related protein Rab34, is localized to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. This protein is involved in protein transport, specifically the redistribution of lysosomes to the peri-Golgi region. In fibroblasts, this protein was colocalized with actin to the membrane ruffles and membranes of relatively large vesicles adjacent to the ruffles.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol 0.1M NaCl,1mM DTT
||31.2 kDa (279aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MNILAPVRRD RVLAELPQCL RKEAALHGHK DFHPRVTCAC QEHRTGTVGF KISKVIVVGD LSVGKTCLIN RFCKDTFDKN YKATIGVDFE MERFEVLGIP FSLQLWDTAG QERFKCIAST YYRGAQAIII VFNLNDVASL EHTKQWLADA LKENDPSSVL LFLVGSKKDL STPAQYALME KDALQVAQEM KAEYWAVSSL TGENVREFFF RVAALTFEAN VLAELEKSGA RRIGDVVRIN SDDSNLYLTA SKKKPTCCP
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.