||SKP2 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 205 amino acids (221-425 a.a.) fused to a GST tag and having a total molecular mass of 49kDa. The SKP2 is purified by proprietary chromatographic techniques.
||SKP2 enzyme is a substrate recognition component of the SCF E3 ubiquitin ligase complex which is involved in the ubiquitination and subsequent proteasomal degradation of target proteins which take part in cell cycle progression, signal transduction and transcription. SKP2 specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SKP2 gene is recognized as a protooncogene regularly involved in the pathogenesis of lymphomas. SKP2 is part of the F-box protein family which is known for its 40 amino acid motif, the F-box. F-box proteins comprise 1 of the 4 subunits of ubiquitin protein ligase complex called SCFs, which play a role in phosphorylation-dependent ubiquitination. There are 3 F-box classes: Fbws having WD-40 domains, Fbls having leucine-rich replicates, and Fbxs having either diverse protein-protein interaction or no recognizable motifs. SKP2 is part of the Fbls class.
||SKP2 is supplied in 50mM Tris-Acetate, pH-7.5, 1mM EDTA and 20% Glycerol.
||Sterile Filtered clear solution.
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
||APC/C-mediated degradation of cell cycle proteins; Adaptive Immunity Signaling; C-MYC pathway; Cell Cycle; Cyclin A:Cdk2-associated events at S phase entry; Delta-Notch Signaling Pathway; FOXM1 transcription factor network; FoxO family signaling; G1/S Transition; Immune System; Notch signaling pathway; Pathways in cancer; Regulation of mitotic cell cycle; p53 pathway; protein binding