Recombinant Tubulin Folding Cofactor A

• Cat.No.: TBCA-4636H
• Product Overview: TBCA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 108 amino acids (1-108 a.a.) and having a molecular mass of 12.8 kDa. The TBCA is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: TBCA-4636H
• Description: TBCA is a tubulin-folding protein which is involved in the early step of the tubulin folding pathway. TBCA is one of four proteins (cofactors A, D, E, and C) implicated in the pathway directing to properly folded beta-tubulin from folding intermediates. Cofactors A and D are thought to be a factor in capturing and stabilizing beta-tubulin in a quasi-native confirmation. TBCA is crucial for cell viability, if reduced it causes a decrease in the amount of soluble tubulin, alterations in microtubules and G1 cell cycle arrest. Cofactor E attaches to the cofactor D-tubulin complex, afterward, interaction with cofactor C triggers the release of tubulin polypeptides that are committed to the native state.
• Source: Human
• Host: Escherichia Coli.
• Form: The TBCA solution contains 20mM Tris-HCl buffer pH 7.5, 1mM DTT and 10% glycerol.
• Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Physical Appearance: Sterile Filtered colorless solution.
• Amino acid sequence: MADPRVRQIK IKTGVVKRLV KEKVMYEKEA KQQEEKIEKM RAEDGENYDI KKQAEILQES RMMIPDCQRR LEAAYLDLQR ILENEKDLEE AEEYKEARLV LDSVKLEA.
• Storage: TBCA although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Pathways: Metabolism of proteins

Gene Information

• Gene Name: TBCA tubulin folding cofactor A [ Homo sapiens ]
• Official Symbol: TBCA
• Synonyms: tubulin folding cofactor A; TBCA; tubulin-specific chaperone a; chaperonin cofactor A; CFA; TCP1-chaperonin cofactor A; Tubulin-folding cofactor A
• Gene ID: 6902
• mRNA Refseq: NM_004607
• Protein Refseq: NP_004598
• MIM: 610058
• UniProt ID: O75347
• Chromosome Location: 5q14.1
• Function: chaperone binding; unfolded protein binding