Active Recombinant Human Peptidylprolyl Isomerase F, His-tagged

• Cat.No.: PPIF-1101H
• Product Overview: Human PPIF (NP_005720, 30 a.a. ~ 207 a.a.) partial recombinant protein expressed in Escherichia coli.

Specification

• Cat. No.: PPIF-1101H
• Description: The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.
• Sequence: MGSSHHHHHHSSGLVPRGSHCSK GSGDPSSSSSSGNPLVYLDVDANG KPLGRVVLELKADVPKTAENFRALC TGEKGFGYKGSTFHRVIPSFMCQA GDFTNHNGTGGKSIYGSRFPDENF TLKHVGPGVLSMANAGPNTNGSQ FFICTIKTDWLDGKHVVFGHVKEGM DVVKKIESFGSKSGRTSKKIVITDCGQLS
• Theoretical MW (kDa): 21
• Form: Liquid
• Preparation Method: Escherichia coli expression system
• Purity: μ 95% by SDS-PAGE
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method).
• Activity: Specific activity is > 120 nmoles/min/ug, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 1 °C in Tris-HCl pH 8.0 using chymotrypsin.
• Application: SDS-PAGE; Functional Study
• Storage Buffer: In 20 mM Tris, pH 7.5 (1 mM dithiothreitol, 10% glycerol).
• Storage: Store at 4°C for 1~2 weeks. For long term storage store at -20°C or -80°C.Aliquot to avoid repeated freezing and thawing.
• Unitprot ID: P30405
• Functions: isomerase activity; peptide binding; peptidyl-prolyl cis-trans isomerase activity

Gene Information

• Gene Name: PPIF peptidylprolyl isomerase F [Homo sapiens]
• Official Symbol: PPIF
• Synonyms: PPIF; peptidylprolyl isomerase F; CYP3; Cyp-D; FLJ90798; MGC117207; peptidylprolyl isomerase F; PPIase; Rotamase; cyclophilin 3; cyclophilin D; cyclophilin F; peptidyl-prolyl cis-trans isomerase, mitochondrial; EC 5.2.1.8; hCyP3; peptidylprolyl isomerase F (cyclophilin F); peptidyl-prolyl cis-trans isomerase, mitochondrial; OTTHUMP00000019925
• Gene ID: 10105
• mRNA Refseq: NM_005729
• Protein Refseq: NP_005720
• MIM: 604486
• Chromosome Location: 10q22-q23