Recombinant Rat Lep

• Cat.No.: Lep-228R

Specification

• Cat. No.: Lep-228R
• Description: Leptin (Greek leptos meaning thin) is a 16 kDa protein hormone that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. The Lep is located on chromosome 7 in humans.
• Source: E. coli
• Species: Rat
• Form: Filtered (0, 4 μm) and lyophilized in 0.5 mg/mL in 0.002 mM NaHCO3
• Research topic: Energy metabolism and body weight regulation
• Purity: >95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: ELISA; Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: VPIHKVQDDT KTLIKTIVTR INDISHTQSV SARQRVTGLD FIPGLHPILS LSKMDQTLAV YQQILTSLPS QNVLQIAHDL ENLRDLLHLL AFSKSCSLPQ TRGLQKPESL DGVLEASLYS TEVVALSRLQ GSLQDILQQL DLSPEC
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: Lep leptin [ Rattus norvegicus ]
• Official Symbol: Lep
• Synonyms: Lep; leptin; OB; obese; obesity factor
• Gene ID: 25608
• mRNA Refseq: NM_013076
• Protein Refseq: NP_037208
• UniProt ID: O00253
• Chromosome Location: 4q22
• Pathway: Adipocytokine signaling pathway; Adipogenesis; Cytokine-cytokine receptor interaction; Diabetes pathways; Incretin Synthesis; Integration of energy metabolism; Jak-STAT signaling pathway; Neuroactive ligand-receptor interaction; Regulation of Insulin Secretion; Synthesis
• Function: cytokine activity; growth factor activity; hormone activity; peptide hormone receptor binding