"Lep" Related Products

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Recombinant Rat Lep

Cat. No. : Lep-228R
Description : Leptin (Greek leptos meaning thin) is a 16 kDa protein hormone that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. The Lep is located on chromosome 7 in humans.
Source : E. coli
Species : Rat
Form : Filtered (0, 4 μm) and lyophilized in 0.5 mg/mL in 0.002 mM NaHCO3
Research topic : Energy metabolism and body weight regulation
Purity : >95%
Reconstitution : Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Applications : ELISA; Western blotting
Quality Control Test : BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
Sequences of amino acids : VPIHKVQDDT KTLIKTIVTR INDISHTQSV SARQRVTGLD FIPGLHPILS LSKMDQTLAV YQQILTSLPS QNVLQIAHDL ENLRDLLHLL AFSKSCSLPQ TRGLQKPESL DGVLEASLYS TEVVALSRLQ GSLQDILQQL DLSPEC
Storage : Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
Leptin :

Gene Name : Lep leptin [ Rattus norvegicus ]
Official Symbol : Lep
Synonyms : Lep; leptin; OB; obese; obesity factor
Gene ID : 25608
mRNA Refseq : NM_013076
Protein Refseq : NP_037208
UniProt ID : O00253
Chromosome Location : 4q22
Pathway : Adipocytokine signaling pathway; Adipogenesis; Cytokine-cytokine receptor interaction; Diabetes pathways; Incretin Synthesis; Integration of energy metabolism; Jak-STAT signaling pathway; Neuroactive ligand-receptor interaction; Regulation of Insulin Secretion; Synthesis
Function : cytokine activity; growth factor activity; hormone activity; peptide hormone receptor binding

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