Recombinant Human CST3, His-tagged

• Cat.No.: CST3-3666H
• Product Overview: Total 129 AA. MW: 14.5 kDa (calculated). N-terminal His-tag, 9 extra AA (highlighted).

Specification

• Cat. No.: CST3-3666H
• Description: Cystatin C is mainly used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. It is a potent inhibitor of lysosomal proteinases and probably one of the most important extracellular inhibitors of cysteine proteases. Cystatin C belongs to the type 2 cystatin gene family.
• Source: E. coli
• Species: Human
• Form: Filtered (0,4 μm) and lyophilized from 0.5 mg/mL in 20mM Tris buffer, 50 mM NaCl, pH 7.5.
• Research topic: Renal disease
• Purity: >95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting; ELISA
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MKHHHHHHAS SP­GKPPRLVG GPMDASVEEE GVRRALDFAV GEYNKASNDM YHSRALQVVR ARKQIVAGVN YFLDVELGRT TCTKTQPNLD NCPFHDQPHL KRKAFCSFQI YAVPWQGTMT LSKSTCQDA
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
• Pathways: Salivary secretion

Gene Information

• Gene Name: CST3 cystatin C [ Homo sapiens ]
• Official Symbol: CST3
• Synonyms: CST3; cystatin C; ARMD11; MGC117328; cystatin 3; cystatin-3; gamma-trace; OTTHUMP00000030440; OTTHUMP00000164181; OTTHUMP00000164182; post-gamma-globulin; bA218C14.4 (cystatin C); neuroendocrine basic polypeptide; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin-C; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin
• Gene ID: 1471
• mRNA Refseq: NM_000099
• Protein Refseq: NP_000090
• MIM: 604312
• UniProt ID: P01034
• Chromosome Location: 20p11.2
• Function: beta-amyloid binding; cysteine-type endopeptidase inhibitor activity; peptidase inhibitor activity; protease binding