"POLB" Related Products

Expand
Expand

Recombinant Human POLB, His-tagged

Cat. No.: POLB-1014H
Product Overview: Recombinant human POLB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-335aa).
Description: DNA polymerase beta, also known as POLB, belongs to the DNA polymerase type-X family. In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. POLB has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. POLB fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells. Overexpression of POLB, as found in some human tumors, could confer an increase in spontaneous mutagenesis.
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
Molecular Weight: 40.3 kDa (355aa), confirmed by MALDI-TOF
Purity: > 90% by SDS - PAGE
Concentration: 0.5 mg/ml (determined by Bradford assay)
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSE
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways: Base excision repair; DNA Repair
Gene Name: POLB polymerase (DNA directed), beta [ Homo sapiens ]
Official Symbol: POLB
Synonyms: POLB; polymerase (DNA directed), beta; MGC125976; DNA polymerase beta; DNA pol beta; DNA polymerase beta subunit; EC 2.7.7.7
Gene ID: 5423
mRNA Refseq: NM_002690
Protein Refseq: NP_002681
MIM: 174760
UniProt ID: P06746
Chromosome Location: 8p11.2
Function: DNA-directed DNA polymerase activity; damaged DNA binding; enzyme binding; lyase activity; metal ion binding; microtubule binding; nucleotidyltransferase activity; protein binding; transferase activity

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.