Recombinant Human POLB, His-tagged
Cat.No. : | POLB-1014H |
Product Overview : | Recombinant human POLB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-335aa). |
- Specification
- Gene Information
- Related Products
Cat. No. : | POLB-1014H |
Description : | DNA polymerase beta, also known as POLB, belongs to the DNA polymerase type-X family. In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. POLB has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. POLB fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells. Overexpression of POLB, as found in some human tumors, could confer an increase in spontaneous mutagenesis. |
Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl |
Molecular Weight : | 40.3 kDa (355aa), confirmed by MALDI-TOF |
Purity : | > 90% by SDS - PAGE |
Concentration : | 0.5 mg/ml (determined by Bradford assay) |
Sequences of amino acids : | MGSSHHHHHH SSGLVPRGSH MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSE |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Pathways : | Base excision repair; DNA Repair |
Gene Name : | POLB polymerase (DNA directed), beta [ Homo sapiens ] |
Official Symbol : | POLB |
Synonyms : | POLB; polymerase (DNA directed), beta; MGC125976; DNA polymerase beta; DNA pol beta; DNA polymerase beta subunit; EC 2.7.7.7 |
Gene ID : | 5423 |
mRNA Refseq : | NM_002690 |
Protein Refseq : | NP_002681 |
MIM : | 174760 |
UniProt ID : | P06746 |
Chromosome Location : | 8p11.2 |
Function : | DNA-directed DNA polymerase activity; damaged DNA binding; enzyme binding; lyase activity; metal ion binding; microtubule binding; nucleotidyltransferase activity; protein binding; transferase activity |
Products Types
◆ Recombinant Protein | ||
POLB-6903M | Recombinant Mouse POLB Protein, His (Fc)-Avi-tagged | +Inquiry |
POLB-3321R | Recombinant Rhesus Macaque POLB Protein, His (Fc)-Avi-tagged | +Inquiry |
POLB-4221R | Recombinant Rat POLB Protein, His (Fc)-Avi-tagged | +Inquiry |
POLB-620H | Recombinant Human Polymerase (DNA Directed), Beta | +Inquiry |
POLB-1521H | Recombinant Human POLB protein | +Inquiry |
◆ Lysates | ||
POLB-1388HCL | Recombinant Human POLB cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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