||Recombinant human CRCP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-148aa).
||CRCP, also known as CGRP-RCP, is an ubiquitous coupling protein for the calcitonin gene-related peptide and adrenomedullin receptors. It modulates ligand responsiveness in a variety of tissues. It has been DNA-directed RNA polymerase activity, catalytic activity and calcitonin receptor activity.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
||19.0 kDa(168aa), confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MEVKDANSAL LSNYEVFQLL TDLKEQRKES GKNKHSSGQQ NLNTITYETL KYISKTPCRH QSPEIVREFL TALKSHKLTK AEKLQLLNHR PVTAVEIQLM VEESEERLTE EQIEALLHTV TSILPAEPEA EQKKNTNSNV AMDEEDPA
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.