||Recombinant human AP1S2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||AP1S2, also known as AP-1 complex subunit sigma-2, serves as the small subunit of AP-1 complex 1 and is a member of the adaptin protein family. Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl, 2mM DTT
||20.7 kDa (177aa), confirmed by MALDI-TOF
||> 85% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MQFMLLFSRQ GKLRLQKWYV PLSDKEKKKI TRELVQTVLA RKPKMCSFLE WRDLKIVYKR YASLYFCCAI EDQDNELITL EIIHRYVELL DKYFGSVCEL DIIFNFEKAY FILDEFLLGG EVQETSKKNV LKAIEQADLL QEEAETPRSV LEEIGLT
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.