||Recombinant mouse S100A8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||S100A8, also known MRP8, is a member of the S100 family, EF-hand superfamily of calcium binding proteins. This protein is localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Altered expression of this protein is associated with the disease cystic fibrosis.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT
||12.4 kDa (109aa), confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||1.0 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MPSELEKALS NLIDVYHNYS NIQGNHHALY KNDFKKMVTT ECPQFVQNIN IENLFRELDI NSDNAINFEE FLAMVIKVGV ASHKDSHKE
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.