Recombinant Human UBE2D1, His-tagged

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Recombinant Human UBE2D1, His-tagged

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Cat. No. : UBE2D1-66H
Product Overview : Recombinant human UBE2D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Description : UBE2D1 belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBE2D1 is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
Source : E. coli.
Form : Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl
Molecular weight : 19.0 kDa (170aa), confirmed by MALDI-TOF
Purity : > 95% by SDS - PAGE
Concentration : 1 mg/ml (determined by Bradford assay)
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MGSMALKRIQ KELSDLQRDP PAHCSAGPVG DDLFHWQATI MGPPDSAYQG GVFFLTVHFP TDYPFKPPKI AFTTKIYHPN INSNGSICLD ILRSQWSPAL TVSKVLLSIC SLLCDPNPDD PLVPDIAQIY KSDKEKYNRH AREWTQKYAM
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDB rendering based on 2c4p. :

Gene Name : UBE2D1 ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast) [ Homo sapiens ]
Synonyms : ubiquitin-conjugating enzyme E2D 1(UBC4/5 homolog, yeast); UBCH5; UBC4/5; UBCH5A; E2(17)KB1; UBC4/5 homolog; stimulator of Fe transport; UBE2D1; ubiquitin-protein ligase D1; ubiquitin carrier protein D1; SFT; ubiquitin-conjugating enzyme E2(17)KB 1; ubiquitin-conjugating enzyme E2-17 kDa 1; SFT; UbcH5A; EC 6.3.2.19; Ubiquitin-conjugating enzyme E2 D1; EC=6.3.2.19
Gene ID : 7321
mRNA Refseq : NM_003338
Protein Refseq : NP_003329
MIM : 602961
UniProt ID : P51668
Chromosome Location : 10q21.1
Function : ATP binding; acid-amino acid ligase activity; ligase activity; protein binding; ubiquitin-protein ligase activity; nucleotide binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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