Mouse anti-HBe Monoclonal Antibody(coating)


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Mouse anti-HBe Monoclonal Antibody(coating)

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Cat. No. : CAB045
Antigen information : Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA(pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA
Specificity : HBe-Ag
Clone : LT03
Host Animal : Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
Immunogen : hepatitis virus granule
Source : Ascites
Format : Purified, Liquid
Purification : >90% pure (SDS-PAGE). Protein A chromatography
Concentration : 4mg/ml (OD280nm, E0.1%= 1.4)
Affinity Constant : 1X1011L/Mol
Buffer : PBS, pH 7.4
Preservative : 0.1% NaN3
Applications : Detection for HBe antigen. Suitable for use in ELISA, Western-Blotting and tissue immunity, Suggested pair for ELISA immunoassay: Capture DetectionCAB045 CAB046
Storage : Short term at 2-8°C,Long term at -20°C
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