Recombinant Human Intestinal Cell (MAK-like) Kinase, GST-tagged

• Cat.No.: ICK-1026H
• Product Overview: Full-length recombinant human ICK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Cat. No.: ICK-1026H
• Description: ICK or intestinal cell kinase belongs to a very extensive family of proteins that share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. ICK encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. ICK localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. ICK purified from transfected human embryonic kidney cells undergoes autophosphorylation and phosphorylates a test protein. ICK plays a key role in development of central nervous, skeletal, and endocrine systems.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 98 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0,25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Specific Activity: 4.8 mmol/min/mg
• Purity: > 75 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: ICK intestinal cell (MAK-like) kinase [ Homo sapiens ]
• Official Symbol: ICK
• Synonyms: ICK; intestinal cell (MAK-like) kinase; ECO; MRK; LCK2; KIAA0936; MGC46090; serine/threonine-protein kinase ICK; hICK; MAK-related kinase; OTTHUMP00000016630; laryngeal cancer kinase 2; serine/threonine protein kinase; NP_055735.1; EC 2.7.11.22; NP_057597.2; Laryngeal cancer kinase 2; Intestinal cell kinase; EC 2.7.11
• Gene ID: 22858
• mRNA Refseq: NM_014920
• Protein Refseq: NP_055735
• MIM: 612325
• UniProt ID: Q9UPZ9
• Chromosome Location: 6p12.1
• Function: ATP binding; cyclin-dependent protein kinase activity; magnesium ion binding; nucleotide binding; protein serine/threonine kinase activity