Recombinant Human Sirtuin 6, His-tagged

• Cat.No.: SIRT6-4882H
• Product Overview: Recombinant human SIRT6 (23-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 23-end a.a.
• Description: SIRT6 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT6 being a member of class IV. Human SIRT6 protein is a NAD (+)-dependent histone H3 lysine-9 deacetylase that modulates telomeric chromatin. SIRT6 associates specifically with telomeres and SIRT6 depletion leads to telomere dysfunction with end-to-end chromosomal fusions and premature cellular senescence.
• Applications: SIRT Assay
• Molecular Weight: 39 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
• Specific Activity: 120 RLU/min/mg
• Purity: > 90 %
• Concentration: 0.1 ug/ul
• Sequences: 23-end
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Signaling events mediated by HDAC Class I

Gene Information

• Gene Name: SIRT6 sirtuin 6 [ Homo sapiens ]
• Official Symbol: SIRT6
• Synonyms: SIRT6; sirtuin 6; SIR2L6; NAD-dependent deacetylase sirtuin-6; sirtuin type 6; SIR2-like protein 6; sir2-related protein type 6; EC 2.4.2.31; sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
• Gene ID: 51548
• mRNA Refseq: NM_016539
• Protein Refseq: NP_057623
• MIM: 606211
• UniProt ID: Q8N6T7
• Chromosome Location: 19p13.3
• Function: NAD binding; NAD (P)+-protein-arginine ADP-ribosyltransferase activity; NAD+ ADP-ribosyltransferase activity; hydrolase activity; metal ion binding; protein binding; transferase activity, transferring glycosyl groups; zinc ion binding; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific)