||Recombinant human PSMB2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||PSMB2 is a member of the proteasome T1B family, which is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 30% glycerol, 0.1 M NaCl.
||24.9 kDa (221 aa) confirmed by MALDI-TOF
||>90 % by SDS-PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLAD CLRSRTPYHV NLLLAGYDEH EGPALYYMDY LAALAKAPFA AHGYGAFLTL SILDRYYTPT ISRERAVELL RKCLEELQKR FILNLPTFSV RIIDKNGIHD LDNISFPKQG S
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||APC/C-mediated degradation of cell cycle proteins; Adaptive Immunity Signaling; Antigen processing; Apoptosis; Assembly of the pre-replicative complex; Autodegradation of the E3 ubiquitin ligase COP1; Cell Cycle Checkpoints; DNA Replication; G1/S DNA Damage Checkpoints; HIV Infection; G1/S Transition; Immune System; Immunoproteasome; Metabolism of RNA