||Recombinant human BDH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||BDH1 is a member of the short-chain dehydrogenase/reductase gene family. This protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. It catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.2M NaCl, 2mM DTT, 0.1mM PMSF.
||35.3 kDa (318aa), confirmed by MALDI-TOF.
||> 85% by SDS-PAGE
||0.25 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MASAAEPVGS KAVLVTGCDS GFGFSLAKHL HSKGFLVFAG CLMKDKGHDG VKELDSLNSD RLRTVQLNVCSSEEVEKVVE IVRSSLKDPE KGMWGLVNNA GISTFGEVEF TSLETYKQVA EVNLWGTVRM TKSFLPLIRR AKGRVVNISS MLGRMANPARSPYCITKFGV EAFSDCLRYE MYPLGVKVSV VEPGNFIAAT SLYSPESIQA IAKKMWEELP EVVRKDYGKK YFDEKIAKME TYCSSGSTDTSPVIDAVTHA LTATTPYTRY HPMDYYWWLR MQIMTHLPGA ISDMIYIR
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Butanoate metabolism; FOXA2 and FOXA3 transcription factor networks; Fatty acid, triacylglycerol, and ketone body metabolism; Ketone body metabolism; Metabolic pathways; Metabolism of lipids and lipoproteins; Synthesis and Degradation of Ketone Bodies;Synthesis of Ketone Bodies; Utilization of Ketone Bodies