Recombinant Human Phosphoglycerate Kinase 2, His-tagged

• Cat.No.: PGK2-981H
• Product Overview: Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl.
• Molecular Weight: 46.9 kDa (437aa), confirmed by MALDI-TOF.
• Purity: > 85% by SDS-PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGVPMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDVYVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNNMEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPLGVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Gluconeogenesis; Glycolysis (Embden-Meyerhof pathway); Glycolysis / Gluconeogenesis; Glycolysis and Gluconeogenesis; Glycolysis; Metabolic pathways

Gene Information

• Gene Name: PGK2 phosphoglycerate kinase 2 [ Homo sapiens ]
• Official Symbol: PGK2
• Synonyms: PGK2; phosphoglycerate kinase 2; PGKB; PGKPS; dJ417L20.2; OTTHUMP00000016591; phosphoglycerate kinase, testis specific; phosphoglycerate kinase autosomal pseudogene; EC 2.7.2.3; PGK-2; Phosphoglycerate kinase, testis specific; dJ417L20.2
• Gene ID: 5232
• mRNA Refseq: NM_138733
• Protein Refseq: NP_620061
• MIM: 172270
• UniProt ID: P07205
• Chromosome Location: 6p12.3
• Function: ATP binding; nucleotide binding; phosphoglycerate kinase activity; transferase activity