||Recombinant human TCEB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||TCEB1 (elongin C) is a subunit of elongin, which is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template encoded arresting sites. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau(VHL) tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing10% glycerol, 1mM DTT, 0.15M NaCl.
||14.6 kDa (132aa), confirmed by MALDI-TOF.
||>90% by SDS-PAGE
||1 mg/ml (determined by Bradford)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MDGEEKTYGG CEGPDAMYVK LISSDGHEFI VKREHALTSG TIKAMLSGPG QFAENETNEV NFREIPSHVL SKVCMYFTYK VRYTNSSTEI PEFPIAPEIA LELLMAANFL DC
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Adaptive Immunity Signaling; Antigen processing: Ubiquitination& Proteasome degradation; Class I MHC mediated antigen processing & presentation; ECS complex; ECV complex; Formation and Maturation of mRNA Transcript; Formation of HIV-1 elongation complex containing HIV-1 Tat; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat; Formation of RNA Pol II elongation complex; Gene Expression; HIF-2-alpha transcription factor network; HIV Infection; HIV Life Cycle; HIV-1 Transcription Elongation; HIV-1 elongation arrest and recovery