||Recombinant human VSTM2L protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||VSTM2L, also known as V-set and transmembrane domain containing 2, is a new modulator of HN neuroprotective activity. It colocalizes with HN in distinct brain areas as well as in primary cultured neurons, where it plays a role in the modulation of neuronal viability. When tested in HN neuroprotection bioassays, it acts as a strong antagonist of HN neuroprotective activity. This protein is the first example of a secreted antagonist of HN and may play a role in the modulation of HN biological functions.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF.
||22.6 kDa (205aa), confirmed by MALDI-TOF.
||>90% by SDS-PAGE
||0.25 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSHMTRPAG HAPWDNHVSG HALFTETPHD MTARTGEDVE MACSFRGSGS PSYSLEIQWW YVRSHRDWTDKQAWASNQLK ASQQEDAGKE ATKISVVKVV GSNISHKLRL SRVKPTDEGS YECRVIDFSD GKARHHKVKA YLRVQPGENS VLHLPEAPPAAPAPPPPKPG KELRKRSVDQ EACSL
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.