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Recombinant Mouse Mif protein

Cat.No. : Mif-5189M
Product Overview : Recombinant Mouse Mif protein was expressed in Escherichia coli.
  • Specification
  • Gene Information
  • Related Products
Description : Macrophage migration inhibitory factor (MIF or MMIF), also named as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is released from white blood cells by bacterial antigen stimulation to trigger an acute immune response, or by glucocorticoids to counter-act the inhibitory effects of glucocorticoids on immune system. MIF is a homotrimer of which each subunit contains 115 amino acids. As mentioned above, MIF is involved in the innate immune response to bacterial pathogens and counter-acts the anti-inflammatory activity of glucocorticoids. Furthermore, it also plays a role as mediator in regulating the function of macrophages in host defense and has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro. Mouse MIF is active on human cells, while human MIF is active on mouse cells. Mouse MIF is 99%, 84%, 90%, and 90% a.a. identical to rat, porcine, bovine and human MIF, respectively.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, 1 mM DTT.
Molecular Mass : Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 115 amino acids.
Protein length : 115
AA Sequence : MPMFIVNTNVPRASVPEGFLSELTQ QLAQATGKPAQYIAVHVVPDQLMTF SGTNDPCALCSLHSIGKIGGAQNRN YSKLLCGLLSDRLHISPDRVYINYY DMNAANVGWNGSTFA
Endotoxin : Less than 1 EU/µg of rMuMIF as determined by LAL method.
Purity : >96% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Mif
Official Symbol : Mif
Synonyms : MIF; macrophage migration inhibitory factor; DER6; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; glycosylation-inhibiting factor; delayed early response protein 6; GIF; Glif; MGC107654;
Gene ID : 17319
mRNA Refseq : NM_010798
Protein Refseq : NP_034928
UniProt ID : P34884

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does MIF contribute to autoimmune diseases? 07/30/2022

MIF can promote inflammation and immune responses, contributing to autoimmune diseases like rheumatoid arthritis and systemic lupus erythematosus.

How does MIF influence neuroinflammation? 10/01/2021

MIF is involved in the regulation of neuroinflammation and has implications in neurodegenerative diseases like Alzheimer's.

What are some conditions where MIF is implicated in clinical applications? 12/22/2019

MIF is implicated in conditions like autoimmune diseases, sepsis, and cancer.

Can MIF be a potential therapeutic target in autoimmune diseases? 08/20/2019

Yes, targeting MIF with drugs or antibodies is being explored as a potential treatment for autoimmune diseases.

What are some other clinical applications of MIF? 02/26/2019

MIF is also studied in the context of cardiovascular diseases, neuroinflammation, and wound healing.

Customer Reviews (3)

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Reviews
12/05/2022

    The cost-effectiveness of the MIF protein, competitive pricing, and the availability of bulk purchase options provided by the manufacturer also align with my budget considerations, allowing me to optimize my resources.

    10/13/2016

      the manufacturer's commitment to delivering the MIF protein in a timely manner is greatly appreciated.

      06/06/2016

        the excellent technical support provided by the manufacturer is of immense value to me.

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