Active Recombinant Staphylokinase (SAK) protein

• Cat.No.: SAK-05S
• Product Overview: Active Recombinant Staphylokinase (SAK) protein(28-163 aa) was expressed in E. coli.

Specification

• Species: Staphylococcus phage phiN315
• Source: E.coli
• Tag: Non
• Protein Length: 28-163 aa
• Form: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.0, 0.02 % Tween80.
• Bio-activity: Fully biologically active when compared to standard. The specific activity determined by fibrining lysis in agarose plate is 5.0 × 10^4 IU/mg.
• Molecular Mass: Approximately 15.6 kDa, a single non-glycosylated polypeptide chain containing 136 amino acids.
• AASequence: SSSFDKGKYK KGDDASYFEP TGPYLMVNVT GVDGKRNELL SPRYVEFPIK PGTTLTKEKI EYYVEWALDA TAYKEFRVVE LDPSAKIEVT YYDKNKKKEE TKSFPITEKG FVVPDLSEHI KNPGFNLITK VVIEKK
• Endotoxin: Less than 1 EU/µg of rSAK as determined by LAL method.
• Purity: > 97% by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.