Recombinant Human HBV Core Protein

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Recombinant Human HBV Core Protein

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Cat. No. : HBcAg-15H
Product Overview : Recombinant Human HBV core protein produced inE. colihas a molecular mass of 16823 Da.
Description : Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.
Purity : ≥95% as determined by SDS-PAGE.
Concentration : 0.2 mg / ml by BCA
Component : HBV core protein, NaHCO310μl, pH 7.4
Specific Activity : Immunoreactive with sera HBV-infected individuals.
Endotoxin : less than 10EU/mg as determined by LAL method
Applications : HBV Core antigen is stable in ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.
Storage buffter : Liquid. In NaHCO3buffer
Storage : HBV Core protein is shipped at ambient temperature. Upon arrival, Store at -20°C . Five years frozen. One month in solution at room temperature.
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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