||Recombinant Human HA, Fc-Tagged expressed in human cells comprises 754 amino acids with the predicted molecular mass of 85.5 kDa. As a result of glycosylation, it migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
||Hemagglutinin (HA) is a Single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. It is a homotrimer of disulfide-linked HA1-HA2. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence. A DNA sequence encoding the extracellular domain of Influenza A virus (A/Anhui/1/2005(H5N1) (ABD28180.1) hemagglutinin (Met 1-Gln 527) with cleavage site mutated (HA1+HA2, uncleaved) was was fused with the C-terminal polyhistidine-tagged Fc region of mouse IgG1 at the C-terminus.
|Predicted N terminal:
||> 97%, as determined by SDS-PAGE
||Supplied as a 0.2μm filtered solution of 100mM Glycine,10mMNaCl,50mMTris,pH7.5
||< 1.0 EU per 1μg cytokine as determined by the LAL method
||Follow the instructions on the vial. Centrifuge the vial at 4°Cbefore opening to recover the entire contents.
||Samples are stable for up to twelve months from date of receipt -70°C
||Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted and be used as soon as possible.Avoid repeated freeze-thaw cycles.