Recombinant Mouse Tumor Necrosis Factor (Ligand) Superfamily, Member 11

• Cat.No.: Tnfsf11-116M
• Product Overview: Recombinant Mouse sRANKL produced in E.coli is single, non-glycosylated, polypeptide chain containing 179 amino acids and having a total molecular mass of 20 kDa. CD254 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: Tnfsf11-116M
• Description: RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein (1mg/ml) was lyophilized with 10mM NaP, pH 7.5+ 50 NaCl.
• Solubility: It is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Ser-Gly-Ala.
• Biological Activity: Measured by its ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 5.0-10.0 ng/ml.
• Storage: Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°Cbetween 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Tnfsf11 tumor necrosis factor (ligand) superfamily, member 11 [ Mus musculus ]
• Synonyms: Tnfsf11; tumor necrosis factor (ligand) superfamily, member 11; ODF; OPG; OPGL; RANKL; Ly109l; Trance; OPG ligand; osteoprotegerin ligand; osteoclast differentiation factor; receptor activator of NF-kappaB ligand; tumor necrosis factor-related activation-induced cytokine; Ly109l
• Gene ID: 21943
• mRNA Refseq: NM_011613
• Protein Refseq: NP_035743
• UniProt ID: O35235
• Chromosome Location: 14 D3; 14 45.0 cM
• Pathway: Cytokine-cytokine receptor interaction
• Function: cytokine activity; protein binding; receptor activity; tumor necrosis factor receptor binding