Recombinant Human Leptin Antagonist Quadruple Mutant

• Cat.No.: LeptinqA-14H
• Product Overview: Recombinant Human Leptin Quadruple Mutant is a single polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a Mw of 16 kDa, Human Leptin was mutated, resulting in L39A/D40A/F41A/I42A. Leptin Antagonist Quadruple Mutant was purified by proprietary chromatographic techniques.

Specification

• Cat. No.: Leptin qA-14H
• Source: Escherichia Coli.
• Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.
• Physical Appearance: White lyophilized (freeze-dried) powder.
• Purity: Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO₃.
• Solubility: It is recommended to reconstitute the lyophilized Leptin Antagonist Quadruple Mutant in sterile 0.4% NaHCO₃adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: ProSpec’s Leptin Quadruple Antagonist Mutant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transected with the long form of human Leptin receptor. It also inhibits various Leptin effects in several in vitro bioassays.
• Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.89 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Storage: Lyophilized Leptin Antagonist Quadruple Mutant although stable at room temperature for several weeks, should be stored desiccated below -18℃. Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 4℃ or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
• Synonyms: OB Protein; Obesity Protein; OBS; Obesity factor; LEP; Lep; leptin; ob; obese; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); FLJ94114; Obese protein.

Gene Information

• Synonyms: OB Protein; Obesity Protein; OBS; Obesity factor; LEP; Lep; leptin; ob; obese; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); FLJ94114; Obese protein.