Recombinant Human Leptin Antagonist Triple Mutant LeptintA-15H

Recombinant Human Leptin Antagonist Triple Mutant

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Recombinant Human Leptin Antagonist Triple Mutant

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Cat. No. : Leptin tA-15H
Product Overview : Recombinant Human Leptin Antagonist Triple Mutant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular weight of 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A. Leptin Antagonist Triple Mutant Recombinant Human was purified by proprietary chromatographic techniques.
Source : Escherichia Coli.
Amino acid sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.
Physical Appearance : White lyophilized (freeze-dried) powder.
Purity : Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
Formulation : The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO3.
Solubility : It is recommended to reconstitute the Recombinant ly Human ophilized Leptin Antagonist Triple Mutant in sterile 0.4% NaHCO3adjusted to pH 8-9, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity : Leptin triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Protein content : Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Storage : Recombinant Human Lyophilized Leptin Antagonist Triple Mutant although stable at room temperature for several weeks, should be stored desiccated below -18℃. Upon reconstitution at > 0.1 mg/ml and up to 2 mM and filter sterilization Leptin mutant can be stored at 4℃or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Synonyms : OB Protein; Obesity Protein; OBS; Obesity factor; LEP; Lep; leptin; ob; obese; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); FLJ94114; Obese protein.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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