Active Recombinant Human Integrin Alpha 5 Beta 3 Heterodimer Protein
Cat.No. : | ITGAVB3-101H |
Product Overview : | Recombinant human Integrin alpha 5 beta 3 heterodimer protein (Human ITGA5 & ITGB3) Phe 42 - Tyr 995 (Integrin alpha 5, NP_002196) and Gly 27 - Asp 718 (Integrin beta 3, AAI27667) was co-expressed in human 293 cells (HEK293). |
- Specification
- Gene Information
- Related Products
Cat. No. : | ITGAVB3-101H |
Description : | The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. |
Source : | HEK293 Cells |
Molecular Mass : | Subunit Integrin alpha 5 (ITGA5) is fused with Strep II-tag at the C-terminus and subunit Integrin beta 3 (ITGB3) is fused with his-tag at the C-terminus. The heterodimer Human ITGA5 & ITGB3, co-expressed by ITGA5 plasmid and ITGB3 plasmid, has a calculated MW of 105.4 kDa ( alpha 5 subunit) and 77.3 kDa (beta 3 subunit). The predicted N-terminus is Phe42 (Integrin alpha 5) and Gly 27 (Integrin beta 3) . DTT-reduced Protein migrates as 105-150 kDa due to glycosylation. |
Formulation : | Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH 7.5 with 150 mM NaCl and 0.5 mM CaCl2. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
Purity : | >90% as determined by SDS-PAGE. |
Bioactivity : | Measured by its ability in functional ELISA. Immobilized recombinant human Vitronectin protein at 1 ug/ml can bind Recombinant human Integrin alpha 5 beta 3 heterodimer protein with apparent KD <8 nM. |
Endotoxin : | Less than 1.0 EU per μg of the Human ITGA5 & ITGB3 by the LAL method. |
Storage : | -20°C to -70°C for 3 months under sterile conditions after reconstitution. Avoid repeated freezethaw cycles. |
OfficialSymbol : | ITGA5 |
Gene Name : | ITGA5 integrin, alpha 5 (fibronectin receptor, alpha polypeptide) [ Homo sapiens ] |
Synonyms : | ITGA5; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); FNRA; CD49e; VLA5A; integrin alpha-5; VLA-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; very late activation protein 5, alpha subunit; Integrin alpha-5; Fibronectin receptor subunit alpha; Integrin alpha-F |
Gene ID : | 3678 |
mRNA Refseq : | NM_002205 |
Protein Refseq : | NP_002196 |
MIM : | 135620 |
UniProt ID : | P08648 |
Chromosome Location : | 12q11-q13 |
Pathway : | Angiopoietin receptor Tie2-mediated signaling; Arrhythmogenic right ventricular cardiomyopathy (ARVC); Axon guidance; Bacterial invasion of epithelial cells; Cell surface interactions at the vascular wall; Dilated cardiomyopathy; ECM-receptor interaction; Focal Adhesion; Focal adhesion; Hematopoietic cell lineage; Hemostasis; Integrin cell surface interactions; Integrin-mediated cell adhesion; L1CAM interactions; Pertussis |
Function : | protein binding; metal ion binding; platelet-derived growth factor receptor binding; vascular endothelial growth factor receptor 2 binding |
Products Types
◆ Recombinant Protein | ||
ITGA5-078H | Recombinant Human ITGA5 Protein, C-His-tagged | +Inquiry |
Itga5-3596M | Recombinant Mouse Itga5 Protein, Myc/DDK-tagged | +Inquiry |
Itga5-1697R | Recombinant Rat Itga5 Protein, His-tagged | +Inquiry |
ITGA5-2341H | Recombinant Human ITGA5 Protein, His-tagged | +Inquiry |
ITGA5-4996H | Recombinant Human ITGA5 Protein | +Inquiry |
◆ Lysates | ||
ITGA5-5132HCL | Recombinant Human ITGA5 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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