||Recombinant S. cerevisiae Translation Associated Element 1/TAE1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1Asn232) of S. cerevisiae TAE1.
||As the initiator Met is cleaved, Alpha Nmethyltransferase melthylates the Nterminus of target proteins containing the N terminal motif [Ala/Pro/Ser]ProLys. In the alphaamino group of Ala or Ser residue of [AlaSer]ProLys motif and mono or dimethylation of Pro in the ProProLys motif, AlphaNmethyltransferase catalyzes mono , di or trimethylation. NTM1 is responsible for the N terminal methylation of ribosomal protein, such as RPL12A, RPL12B, RPS25A, RPS25B.
||Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4
||Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
||For research use only.
||Lyophilized protein should be stored at less than 20°C. though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C. for 2-7 days.Aliquots of reconstituted samples are stable at less than 20°C. for 3 months.