||Alanine Aminotransferase Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 54,479 Dalton.The ALT is purified by chromatographic techniques.
||Alanine transaminase?or?ALT?is a?transaminase?enzyme?(EC?188.8.131.52). It is also called?serum glutamic pyruvic transaminase(SGPT) or?alanine aminotransferase?(ALAT).ALT is found in?serum?and in various bodily tissues, but is most commonly associated with the?liver. It catalyzes the two parts of thealanine cycle.
||The protein was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5"-phosphate.
||The specific activity was found to be 1,000 U/mg.
||Greater than 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
||For research use only.
||AAT1 although stable at 10 ℃ for days, should be stored desiccated below -18 ℃. Please prevent freeze/thaw cycles.