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Recombinant Human Glypican 1, His-tagged

Cat.No.: GPC1-152H
ProductOverview: Recombinant Human Glypican 1(Asp24-Ser530), fused with a C-terminal 6-His tag, was expressed in Mouse NSO Cells.
Description: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
Species: Human
Source: Mouse NSO Cells
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Purity: > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rhGlypican 1 at 3 µg/mL (100 µL/well) will bind rhFGF-basic with an apparent KD < 1 nM.
EndotoxinLevel: < 0.10 EU per 1 μg of the protein by the LAL method.
PredictedN terminal: Asp24
MolecularMass: The recombinant protein has a calculated Molecular Mass of 56.8 kDa, In SDS-PAGE under reducing conditions the apparent molecular mass of the protein is approximately 60 kDa.
Reconstitution: Reconstitute at 500 µg/mL in sterile PBS.
Storage: Store it under sterile conditions at -20 ~ -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Gene Name: GPC1 glypican 1 [ Homo sapiens ]
Official Symbol: GPC1
Synonyms: GPC1; glypican 1; glypican-1; glypican; glypican proteoglycan 1
Gene ID: 2817
mRNA Refseq: NM_002081
Protein Refseq: NP_002072
MIM: 600395
UniProt ID: P35052
Chromosome Location: 2q35-q37
Pathway: Activation of Rac, organism-specific biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Glypican 1 network, organism-specific biosystem; Glypican pathway, organism-specific biosystem
Function: heparan sulfate proteoglycan binding

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