Active Recombinant Human Chordin-Like 1, His-tagged

• Cat.No.: CHRDL1-3189H
• Product Overview: Recombinant Human CHRDL1 (Glu22-Cys450) fused with a C­terminal and 10­His tag, was expressed in Mouse NSO Cells.

Specification

• Cat. No.: CHRDL1-3189H
• Description: This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described.
• Source: Mammalian cells
• Molecular Mass: The protein has a calculated molecular mass of 50 kDa. The apparent molecular mass of the recombinant protein is approximately 60 KDa in SDS-PAGE under reducing conditions.
• Predicted N terminal: Glu22
• Formulation: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Activity: Measured by its ability to inhibit BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is typically 0.7 - 3.5 µg/mL in the presence of 50 ng/mL rhBMP-4 and 1 µg/mL of rmTSG.
• Reconstitution: Reconstitute at 200 µg/mL in sterile 4 mM HCl.
• EndotoxinLevel: < 0.01 EU per 1 μg of the protein by the LAL method.
• Storage: 12 months from date of receipt, ­20 to ­70 °C as supplied. Avoid repeated freeze­thaw cycles.
• OfficialSymbol: CHRDL1

Gene Information

• Gene Name: CHRDL1 chordin-like 1 [ Homo sapiens ]
• Synonyms: chordin-like 1; CHL; VOPT; NRLN1; dA141H5.1; CHRDL1; chordin-like protein 1; neuralin 1; neuralin-1; ventroptin; neurogenesin-1; OTTHUMP00000023853; OTTHUMP00000023854; OTTHUMP00000216338; Neurogenesin-1; Ventroptin; Neuralin-1
• Gene ID: 91851
• mRNA Refseq: NM_145234
• Protein Refseq: NP_660277
• MIM: 300350
• UniProt ID: Q9BU40
• Chromosome Location: Xq23
• Pathway: Signaling by BMP; Signal Transduction
• Function: BMP signaling pathway; cell differentiation; multicellular organismal development; nervous system development; ossification