Recombinant Human Chordin-Like 1, His-tagged CHRDL1-3189H

Recombinant Human Chordin-Like 1, His-tagged

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Recombinant Human Chordin-Like 1, His-tagged

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Cat. No. : CHRDL1-3189H
Product Overview : Recombinant Human CHRDL1 (Glu22-Cys450) fused with a C­terminal and 10­His tag, was expressed in Mouse NSO Cells.
Description : This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described.
Source : Mouse NSO Cells
Molecular Mass : The protein has a calculated molecular mass of 50 kDa. The apparent molecular mass of the recombinant protein is approximately 60 KDa in SDS-PAGE under reducing conditions.
Predicted N terminal : Glu22
Formulation : Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Activity : Measured by its ability to inhibit BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is typically 0.7 - 3.5 µg/mL in the presence of 50 ng/mL rhBMP-4 and 1 µg/mL of rmTSG.
Reconstitution : Reconstitute at 200 µg/mL in sterile 4 mM HCl.
EndotoxinLevel : < 0.01 EU per 1 μg of the protein by the LAL method.
Storage : 12 months from date of receipt, ­20 to ­70 °C as supplied. Avoid repeated freeze­thaw cycles.
OfficialSymbol : CHRDL1
Gene Name : CHRDL1 chordin-like 1 [ Homo sapiens ]
Synonyms : chordin-like 1; CHL; VOPT; NRLN1; dA141H5.1; CHRDL1; chordin-like protein 1; neuralin 1; neuralin-1; ventroptin; neurogenesin-1; OTTHUMP00000023853; OTTHUMP00000023854; OTTHUMP00000216338; Neurogenesin-1; Ventroptin; Neuralin-1
Gene ID : 91851
mRNA Refseq : NM_145234
Protein Refseq : NP_660277
MIM : 300350
UniProt ID : Q9BU40
Chromosome Location : Xq23
Pathway : Signaling by BMP; Signal Transduction
Function : BMP signaling pathway; cell differentiation; multicellular organismal development; nervous system development; ossification

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