Active Recombinant Aequorea victoria GFP protein, His-tagged

• Cat.No.: GFP-03A
• Product Overview: Recombinant Aequorea victoria GFP protein(AAB65663)(Ser2-Lys238), fused with His tag, was expressed in E. coli.

Specification

• Species: Aequorea victoria
• Source: E.coli
• Tag: His
• Protein Length: Ser2-Lys238
• Form: Lyophilized from sterile PBS, pH7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Activity Assay
• Molecular Mass: The recombinant Aequorea victoria GFP consisting of 253 amino acids and has a calculated molecular mass of 28.7 kDa. It migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions as predicted.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Evolution of structure-function relationships in the GFP-family of proteins. (2014)