||Recombinant Human Interleukin-8 produced inE.Coliis single, a non-glycosylated, Polypeptide chain containing 77 amino acids fragment (23-99) and having a total molecular mass of 15kDa with an amino-terminal his-tagged. The IL-8 His is purified by proprietary chromatographic techniques.
||Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. When first encountering an antigen, the primary cells to encounter it are the macrophages who phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor.
||Sterile Filtered clear solution.
||Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
||IL8 His (0.17mg/ml) is supplied in 1x PBS and 50% glycerol.
||Specific Activity of IL8 in chemotaxis of donor PBL neutrophils, threshold concentration corresponding to 25ng/ml.
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.