Active Recombinant Human GCK, His-tagged

• Cat.No.: GCK-200H
• Product Overview: The recombinant Human GCK, His-tagged protein(isoform 3) was expressed in E. coli and purified by affinity chromatography in combination with FPLC columns.

Specification

• Description: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
• Source: E. coli
• Species: Human
• Tag: His
• Form: The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
• Bio-activity: 1 unit equals 1 nanogram of purified protein.
• Molecular Mass: 53.0 kDa
• AA Sequence: MPRPRSQLPQ PNSQVEQILA EFQLQEEDLK KVMRRMQKEM DRGLRLETHE EASVKMLPTY VRSTPEGSEV GDFLSLDLGG TNFRVMLVKV GEGEEGQWSV KTKHQMYSIP EDAMTGTAEM LFDYISECIS DFLDKHQMKH KKLPLGFTFS FPVRHEDIDK GILLNWTKGF KASGAEGNNV VGLLRDAIKR RGDFEMDVVA MVNDTVATMI SCYYEDHQCE VGMIVGTGCN ACYMEEMQNV ELVEGDEGRM CVNTEWGAFG DSGELDEFLL EYDRLVDESS ANPGQQLYEK LIGGKYMGEL VRLVLLRLVD ENLLFHGEAS EQLRTRGAFE TRFVSQVESD TGDRKQIYNI LSTLGLRPST TDCDIVRRAC ESVSTRAAHM CSAGLAGVIN RMRESRSEDV MRITVGVDGS VYKLHPSFKE RFHASVRRLT PSCEITFIES EEGSGRGAAL VSAVACKKAC MLGQ
• Purity: Greater than 95% as determined by SDS-PAGE.
• Applications: Can be used for the phosphorylation of Glucose, assay development.
• Storage: Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Gene Information

• Gene Name: GCK glucokinase (hexokinase 4) [ Homo sapiens ]
• Official Symbol: GCK
• Synonyms: GCK; glucokinase (hexokinase 4); maturity onset diabetes of the young 2 , MODY2; glucokinase; HK4; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; hexokinase D, pancreatic isozyme; ATP:D-hexose 6-phosphotransferase; GK; GLK; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
• Gene ID: 2645
• mRNA Refseq: NM_033508
• Protein Refseq: NP_000153
• MIM: 138079
• UniProt ID: P35557
• Chromosome Location: 7p15.3-p15.1
• Pathway: Amino sugar and nucleotide sugar metabolism, organism-specific biosystem; Amino sugar and nucleotide sugar metabolism, conserved biosystem; Butirosin and neomycin biosynthesis, organism-specific biosystem; Butirosin and neomycin biosynthesis, conserved biosystem; Developmental Biology, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; GDP-glucose biosynthesis, organism-specific biosystem
• Function: ATP binding; glucokinase activity; glucose binding; kinase activity; nucleotide binding; protein binding; transferase activity