Recombinant Mouse Csf3 protein Csf3-100M

Recombinant Mouse Csf3 protein

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Recombinant Mouse Csf3 protein

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Cat.No. : Csf3-100M
Product Overview : Recombinant Mouse Csf3 protein was expressed in Escherichia coli.
Description : The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered concentrated solution in 10 mM Sodium Citrate, pH 4.0, 150 mM NaCl, 0.01 % Tween-20.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg.
Molecular Mass : Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 178 amino acids.
Protein length : 178
AA Sequence : VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA
Endotoxin : Less than 0.1 EU/μg of rMuG-CSF as determined by LAL method.
Purity : >98% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
SDS-PAGE of Csf3-100M :

Gene Name : Csf3
Official Symbol : Csf3
Synonyms : CSF3; colony stimulating factor 3 (granulocyte); granulocyte colony-stimulating factor; Csfg; G-CSF; MGI-IG;
Gene ID : 12985
mRNA Refseq : NM_009971
Protein Refseq : NP_034101
UniProt ID : P09920
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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