Recombinant Human ASAP2, His-tagged

• Cat.No.: ASAP2-9911H
• Product Overview: Recombinant Human ASAP2 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Description: This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: E.coli
• Species: Human
• Tag: His
• Protein length: 1-357a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: ASAP2 ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 [ Homo sapiens ]
• Official Symbol: ASAP2
• Synonyms: ASAP2; ArfGAP with SH3 domain, ankyrin repeat and PH domain 2; DDEF2, development and differentiation enhancing factor 2; arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2; centaurin; beta 3; CENTB3; KIAA0400; PAP; SHAG1; centaurin, beta 3; PYK2 C terminus-associated protein; pyk2 C-terminus-associated protein; development and differentiation enhancing factor 2; development and differentiation-enhancing factor 2; paxillin-associated protein with ARF GAP activity 3; PAG3; AMAP2; DDEF2; Pap-alpha; FLJ42910
• Gene ID: 8853
• mRNA Refseq: NM_001135191
• Protein Refseq: NP_001128663
• MIM: 603817
• UniProt ID: O43150
• Chromosome Location: 2p24
• Pathway: Arf6 trafficking events, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem
• Function: ARF GTPase activator activity; enzyme activator activity; metal ion binding; protein binding; zinc ion binding