Recombinant Human ATP6V1F protein(Met1-Arg119), GST-tagged

• Cat.No.: ATP6V1F-10048H
• Product Overview: Recombinant Human ATP6V1F (NP_004222.2) (Met1-Arg119) was expressed in E. coli with the GST tag at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: N-GST
• Protein length: Met1-Arg119
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human ATP6V1F/GST chimera consists of 350 amino acids and has a predicted molecular mass of 40.2 KDa. It migrates as an approximately 40 KDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ATP6V1F ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F [ Homo sapiens ]
• Official Symbol: ATP6V1F
• Synonyms: ATP6V1F; ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F; V-type proton ATPase subunit F; ATP6S14; VATF; Vma7; V-ATPase F subunit; V-ATPase subunit F; ATPase, vacuolar, 14 kD; V-ATPase 14 kDa subunit; vacuolar proton pump F subunit; vacuolar proton pump subunit F; vacuolar ATP synthase subunit F; adenosinetriphosphatase 14k chain; H(+)-transporting two-sector ATPase, 14kD subunit; MGC117321; MGC126037; MGC126038
• Gene ID: 9296
• mRNA Refseq: NM_001198909
• Protein Refseq: NP_001185838
• MIM: 607160
• UniProt ID: Q16864