Recombinant Human CARS, His-tagged CARS-10724H

Recombinant Human CARS, His-tagged


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Recombinant Human CARS, His-tagged

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Cat.No. : CARS-10724H
Product Overview : Recombinant Human CARS protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.
Description : Breakdown products of phosphoinositides are ubiquitous second messengers that function downstream of many G protein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase C activity. This gene encodes an enzyme which regulates the amount of phosphatidylinositol available for signaling by catalyzing the conversion of phosphatidic acid to CDP-diacylglycerol. This enzyme is an integral membrane protein localized to two subcellular domains, the matrix side of the inner mitochondrial membrane where it is thought to be involved in the synthesis of phosphatidylglycerol and cardiolipin and the cytoplasmic side of the endoplasmic reticulum where it functions in phosphatidylinositol biosynthesis. Two genes encoding this enzyme have been identified in humans, one mapping to human chromosome 4q21 and a second to 20p13.
Source : E.coli
Species : Human
Tag : His
Protein length : 1-353a.a.
Storage : The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
Storage Buffer : 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.
Gene Name : CARS cysteinyl-tRNA synthetase [ Homo sapiens ]
Official Symbol : CARS
Synonyms : CARS; cysteinyl-tRNA synthetase; cysteine--tRNA ligase, cytoplasmic; CARS1; cysteine tRNA ligase 1; cytoplasmic; cysteine translase; cysteine tRNA ligase 1, cytoplasmic; CYSRS; MGC:11246;
Gene ID : 833
mRNA Refseq : NM_001014437
Protein Refseq : NP_001014437
MIM : 123859
UniProt ID : P49589
Chromosome Location : 11p15.5
Pathway : Aminoacyl-tRNA biosynthesis, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conserved biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; Cytosolic tRNA aminoacylation, organism-specific biosystem; Gene Expression, organism-specific biosystem; tRNA Aminoacylation, organism-specific biosystem;
Function : ATP binding; ATP binding; cysteine-tRNA ligase activity; cysteine-tRNA ligase activity; cysteine-tRNA ligase activity; ligase activity; metal ion binding; nucleotide binding; protein binding; protein homodimerization activity; protein homodimerization act

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