Recombinant Human CRYBB1, His-tagged CRYBB1-11601H

Recombinant Human CRYBB1, His-tagged

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Recombinant Human CRYBB1, His-tagged

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Cat.No. : CRYBB1-11601H
Product Overview : Recombinant Human CRYBB1 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.
Description : Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta basic group member, undergoes extensive cleavage at its N-terminal extension during lens maturation. It is also a member of a gene cluster with beta-A4, beta-B2, and beta-B3.
Source : E.coli
Species : Human
Tag : His
Protein length : 1-252a.a.
Storage : The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
Storage Buffer : 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.
Gene Name : CRYBB1 crystallin, beta B1 [ Homo sapiens ]
Official Symbol : CRYBB1
Synonyms : CRYBB1; crystallin, beta B1; beta-crystallin B1; beta-B1 crystallin; eye lens structural protein; CATCN3;
Gene ID : 1414
mRNA Refseq : NM_001887
Protein Refseq : NP_001878
UniProt ID : P53674
Chromosome Location : 22q12.1
Function : structural constituent of eye lens;

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