||Recombinant Human DBR1 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.
||The protein encoded by this gene is an RNA lariat debranching enzyme that hydrolyzes 2-5 prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication.
||The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
||1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.