|Product Overview:||Recombinant Human ENOX2(292 - 610 aa) fused with GST tag was expressed in E. coli.|
|Description:||This gene is a tumor-specific member of the ECTO-NOX family of genes that encode cell surface NADH oxidases. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The protein also displays prion-like properties. Alternative splicing results in multiple transcript variants.|
|Form:||Protein lyophilized in sterile PBS (58 mM Na2HPO4, 17 mM NaH2PO4, 68 mM NaCl, 300 mM Imidazole, pH 8.0). Trehalose (5-8%) and mannitol (5-8%) protectants were added before lyophilization.|
|Protein length:||292 - 610 aa|
|Purity:||90%, by SDS-PAGE with Coomassie Brilliant Blue staining.|
|Storage:||Store for up to 12 months at -20 centigrade to -80 centigrade as lyophilized powder.
Storage of Reconstituted Protein:
Short-term storage: Store at 2-8 centigrade for two weeks.
Long-term storage: Aliquot and store at -20 centigrade to -80 centigrade for up to 6 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
|Reconstitution:||Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below).|
|Gene Name:||ENOX2 ecto-NOX disulfide-thiol exchanger 2 [ Homo sapiens ]|
|Synonyms:||ecto-NOX disulfide-thiol exchanger 2; 2259; Ensembl:ENSG00000165675; ecto-NOX disulfide-thiol exchanger 2;APK1 antigen;cytosolic ovarian carcinoma antigen 1;tumor-associated hydroquinone oxidase; APK1; tNOX; COVA1|
|Function:||nucleic acid binding; nucleotide binding; protein disulfide oxidoreductase activity;|