Recombinant Bovine Growth Hormone Receptor

• Cat.No.: GHR-36B
• Product Overview: Recombinant Bovine Growth Hormone Binding Protein produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 237 amino acids and having a molecular mass of 28 kDa. Bovine GHBP is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: GHR-36B
• Description: GHBP is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. A common alternate allele of this gene, called GHRd3, lacks exon three and has been well-characterized. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. Other splice variants, including one encoding a soluble form of the protein (GHRtr), have been observed but have not been thoroughly characterized.
• Source: E.Coli.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Ser-Gly-Ser.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The Protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO₃.
• Solubility: It is recommended to reconstitute the lyophilized GHBP in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Lyophilized Growth Hormone Binding Protein Bovine although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution GHBP should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: GHR growth hormone receptor [ Bos taurus ]
• Synonyms: GHR; growth hormone receptor; GHBP; serum binding protein; somatotropin receptor; growth hormone binding protein; GH receptor; Somatotropin receptor
• Gene ID: 280805
• mRNA Refseq: NM_176608
• Protein Refseq: NP_788781
• UniProt ID: O46600
• Pathway: Jak-STAT signaling pathway; Neuroactive ligand-receptor interaction; Cytokine-cytokine receptor interaction
• Function: growth hormone receptor activity; peptide hormone binding; phosphate binding; protein binding; protein homodimerization activity; receptor activity